Universidad Peruana Cayetano Heredia  

Microscopic Observation Drug Susceptibility Assay

Middlebrook 7H9 Broth

with Casitone and Glycerol
Flowchart: Alternate Process: 3.1ml glycerol + 1.25g Bacto Casitone Flowchart: Alternate Process: Resuspend 5.9g 7H9 media powder Flowchart: Alternate Process: Autoclave at 121-124oC for 15 min Flowchart: Alternate Process: Divide into 4.5ml aliquots, incubate at 37oC for 48 hours Flowchart: Alternate Process: Store at 6-8oC for up to 1 month

Begin with 900ml deionized H2O

Lack of turbidity signifies a sterile solution
Flowchart: Alternate Process: Day of Use: Add 0.5ml OADC