Universidad Peruana Cayetano Heredia��

Microscopic Observation Drug Susceptibility Assay

Antibiotic Working Solution Preparation

 

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Flowchart: Alternate Process: Thaw aliquot* of INH storage stock and add 100�l to 900�l of 7H9/OADC Flowchart: Alternate Process: Add 1ml of 40�g/ml to 9ml of 7H9/OADC (gives 10ml of 4�g/ml) Flowchart: Alternate Process: Plating 100�l of 4 �g/ml to 900�l 7H9/OADC yields final concentration of 0.4�g/ml Flowchart: Alternate Process: Plating 100�l of 10 �g/ml to 900�l 7H9/OADC yields final concentration of 1�g/ml Flowchart: Alternate Process: Add 1ml of 100�g/ml to 9ml of 7H9/OADC (gives 10ml of 10�g/ml) Flowchart: Alternate Process: Thaw aliquot* of RIF storage stock and add 100�l to 900�l of 7H9/OADC

STOCK No. 2

40�g/ml

STOCK No. 2

100�g/ml

Working Solution

*Once thawed, unused antibiotic must be discarded as properties may not be retained

Isoniazid

Rifampicin