Universidad Peruana Cayetano Heredia��

Microscopic Observation Drug Susceptibility Assay

Preparation of

Sputum Decontamination

Solution (200ml)

100ml sterile deionized H2O

100ml sterile deionized H2O
Flowchart: Alternate Process: 4.0g NaOH Flowchart: Alternate Process: Day of Use: Add 0.1g NALC crystals to every 20ml of decontamination solution Flowchart: Alternate Process: Store at 6-8oC for up to 1 month Flowchart: Alternate Process: Autoclave 121-124oC for 15 min Flowchart: Alternate Process: 2.9g Sodium Citrate

Discard any unused solution after 24 hours