Universidad Peruana Cayetano Heredia��

Microscopic Observation Drug Susceptibility Assay

Plating broth, sample, and antibiotics
Flowchart: Alternate Process: Resuspend pellet in 5ml final 7H9 medium Flowchart: Alternate Process: Aliquot 900μl of broth/sample to 4 wells of a 24 well plate Flowchart: Alternate Process: Add 0.1ml PANTA

Add 100μl INH

Add 100μl Rif

100μl 7H9 with OADC